A resonance light-scattering determination of proteins with fast green FCF.

نویسندگان

  • Yuan Fang Li
  • Cheng Zhi Huang
  • Ming Li
چکیده

The interaction of Fast Green FCF (FCF) with proteins (including bovine serum albumin (BSA), human serum albumin (HSA), pepsin (Pep) and alpha-chymotrypsin (Chy), and lysozyme (Lys)) was characterized by enhanced resonance light-scattering (RLS) measurements using a common spectrofluorometer. The enhanced RLS signals of FCF by proteins at 279.0 nm were obtained, and the mechanism of the RLS enhancement was considered in terms of the effects of the pH and ionic strength on the interaction. It was found that the enhanced RLS intensities were in proportion to the concentrations of proteins in the range of nanogram levels, displaying that the present assay is much more sensitive than the reported RLS methods, with the limits of determination being 4.54, 0.6, 22.8, 4.32 and 1.75 ng/ml for BSA, HSA, Pep, Chy, and Lys. respectively.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Determination of proteins with Fast Red VR by a corrected resonance light-scattering technique.

A simple corrected resonance light-scattering (CRLS) technique was established to correct for any distortion of the resonance light scattering (RLS) spectra resulting from molecular absorption. By using an absorption cell holder to change the propagation direction of the incident light beam of a common spectrofluorometer, the molecular absorption was directly measured through a spectrofluoromet...

متن کامل

Determination of Sulfasalazine in Sulfasalazine Tablets Using Silver Nanoparticles

     Resonance light scattering (RLS) intensity of silver nanoparticles in the presence of sodium dodecylsulphate was quenched on addition of sulfasalzine. The quenching extent was shown to be proportional to the sulfasalazine concentration. Effect of different parameters such as pH, volume and kind of buffer, silver nanoparticles concentration, sodium dodecylsulphate (SDS) concentration, and w...

متن کامل

Microwave-assisted green synthesis of Gold nanoparticles and Its catalytic activity

The present work demonstrated a green approach of synthesis of gold nanoparticles using Eupatorium odoratum leaf extract as reducing and stabilizing agent assisted with microwave irradiation. Effects of various concentrations of leaf extract on the preparation of gold nanoparticles have been investigated and it was monitored by undertaking UV-vis spectroscopic studies. The experimental results ...

متن کامل

Identification and Determination of the Number of Green Citrus Fruit under Different Ambient Light Conditions

Yield mapping by machine aided harvesting requires automatic detection and counting of fruit in a tree canopy. However, occlusion, varying illumination, and similarity with the background make fruit identification a very challenging task. Moreover, green citrus detection within green canopy is a very difficult problem due to the issues previously mentioned. In this study, a novel and simple tec...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

دوره 18 2  شماره 

صفحات  -

تاریخ انتشار 2002